Calculate a Pfaffl expression ratio from supplied Ct values and amplification ef
Calculate a Pfaffl expression ratio from supplied Ct values and amplification efficiencies; require a declared stable reference. No diagnostic interpretation. New modular adapter: retained result recovery for 24 hours, minimal receipt metadata for 90 days. Existing live route unchanged.
Answers HTTP 429 without x402 payment termsour last check, 2026-10-18
0 of 1checks answered this week
n/amedian answer time
$0.010043listed price per call
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Paid test badge: not yet. The checks above are free: we call the tool without paying and read the payment request it sends back. The Verified badge needs paid calls whose answers match the promised output, and nobody can buy a badge.
Endpoint
POST https://www.mahastrategies.com/api/v1/compute/biology/qpcr-relative-expression
| Category | Everything else |
|---|---|
| Provider host | www.mahastrategies.com |
| Networks | eip155:8453 |
| Payment schemes | exact |
| Self-reported calls, 30 days | 1 from 1 payers (the provider's figure, not ours) |
Our checks, last 30 days
| Day | Result | HTTP | Asked | Time |
|---|---|---|---|---|
| 2026-10-18 | no x402 terms | 429 | 16 ms |
Example input (from the provider)
{
"body": {
"dataClass": "synthetic",
"referenceControlCt": 18,
"referenceEfficiency": 1,
"referenceSampleCt": 18,
"referenceStable": true,
"targetControlCt": 22,
"targetEfficiency": 1,
"targetSampleCt": 20
},
"bodyType": "json",
"method": "POST",
"type": "http"
}
Promised output schema (from the provider)
{
"$schema": "https://json-schema.org/draft/2020-12/schema",
"properties": {
"input": {
"additionalProperties": false,
"properties": {
"body": {
"additionalProperties": false,
"properties": {
"dataClass": {
"enum": [
"public",
"synthetic"
],
"type": "string"
},
"referenceControlCt": {
"maximum": 1000000000000,
"minimum": -1000000000000,
"type": "number"
},
"referenceEfficiency": {
"maximum": 1000000000000,
"minimum": -1000000000000,
"type": "number"
},
"referenceSampleCt": {
"maximum": 1000000000000,
"minimum": -1000000000000,
"type": "number"
},
"referenceStable": {
"type": "boolean"
},
"targetControlCt": {
"maximum": 1000000000000,
"minimum": -1000000000000,
"type": "number"
},
"targetEfficiency": {
"maximum": 1000000000000,
"minimum": -1000000000000,
"type": "number"
},
"targetSampleCt": {
"maximum": 1000000000000,
"minimum": -1000000000000,
"type": "number"
}
},
"required": [
"dataClass",
"targetSampleCt",
"targetControlCt",
"referenceSampleCt",
"referenceControlCt",
"targetEfficiency",
"referenceEfficiency",
"referenceStable"
],
"type": "object"
},
"bodyType": {
"enum": [
"json",
"form-data",
"text"
],
"type": "string"
},
"method": {
"enum": [
"POST"
],
"type": "string"
},
"type": {
"const": "http",
"type": "string"
}
},
"required": [
"type",
"method",
"bodyType",
"body"
],
"type": "object"
},
"output": {
"properties": {
"example": {
"additionalProperties": false,
"properties": {
"data": {
"type": "object"
},
"proof": {
"type": "object"
},
"success": {
"const": true
}
},
"required": [
"success",
"data",
"proof"
],
"type": "object"
},
"type": {
"type": "string"
}
},
"required": [
"type"
],
"type": "object"
}
},
"required": [
"input"
],
"type": "object"
}